Technology Description

Professor Seongjin Seo at the University of Iowa has developed novel mutant lox sequences that can be used in a series of AAV vectors, which enable the assembly of multiple AAV vectors in a pre-designed configuration using the CRE-lox recombination system. This method enables the delivery of large genes that exceed the AAV’s packaging capacity, split into up to four AAV vectors, and may be used for (but not limited to) gene therapies requiring large gene delivery. Using this novel approach will help with the delivery of genes that may be crucial for gene therapies that are currently unable to be packaged and delivered to target organs. 

Advantages

  • An order of magnitude higher gene reconstitution efficiencies compared to conventional, spontaneous recombination methods  

  • Little restriction on split site selection 

  • Up to four AAV vectors can be assembled in a predetermined configuration

IP

Patent pending. 

Inventors
To learn more about this technology, please contact Kellen Sensor about UIRF Case No. 2023-011.